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Custom antibody service

Work
Price
Time taken
1.   Antigen expression in bacteria using customer constructed plasmid and purification of 2-3 mg antigen. Work includes transformation of the plasmid into E. coli, expression and purification of the antigen, SDS-PAGE of the purified antigen.
$500 US
It depends on how difficult the purification goes. Usually it takes 10 days to 1 month.
2.   Peptide synthesis
 
Please see Peptide price
2-3 weeks for peptides less than 20 residues and 3-4 weeks for peptides having 21 to 30 residues.
3.   Coupling 2-5 mg peptide onto carrier protein
$100 US
3 days
4.   Rat monoclonal antibody
Depends on customer choices
About 17-22 weeks
5.   Murine monoclonal antibody

Depends on customer choices
About 17-22 weeks
6.   Storage cells in liquid nitrogen
$0.06 US per vial per day
 
7.   Thaw and grow cells from liquid nitrogen
$30 US/vial
About 5 days
8.   Polyclonal anti-serum from one rabbit
$400 US
About 6-10 weeks
9.   Polyclonal anti-serum from one rat 
$300 US
About 6-10 weeks
10. Purification of 5 mg IgG monoclonal antibody from culture supernatant. Work includes cell culture, antibody purification by protein G or protein A columns, ELISA and SDS-PAGE for purified antibody.
$400 US
About 3 weeks
11. Purification of 5 mg IgA or IgM monoclonal antibody from culture supernatant. Work includes cell culture, preparation of antigen coupled Sepharose beads or using protein L beads, antibody purification, ELISA and SDS-PAGE for purified antibody.
$600 US
About 3 weeks
12. Affinity purification of 5 mg polyclonal antibody from antiserum. Work includes preparation of antigen coupled Sepharose beads, antibody affinity purification, ELISA and SDS-PAGE for purified antibody.
$400 US
One week
13. Tandem affinity purification using two antigen columns to purify polyclonal antibody from antiserum
$600 US
One week
14. 10 mg antibody digestion to Fab fragments by papain and absorbance of undigested antibody using protein A or protein G columns. ELISA and SDS-PAGE for digested antibody.
$700 US
One week
15. 10 mg antibody digestion to F(ab')2 fragments by pepsin and absorbance of undigested antibody using protein A or protein G columns. ELISA and SDS-PAGE for digested antibody.
$550 US
One week
16.  FITC labelling of 5 mg antibody
$100 US
3 days
17.  Peroxidase labelling of 5 mg antibody
$300 US
3 days
18.  Biotin labelling of 5 mg antibody
$100 US
3 days
19. Sequencing antibody variable regions.
Work includes isolating RNA from hybridomas, trancribing into cDNA, PCRing using various primers, ligating PCR products onto a vector, transforming bacteria and finally sequencing.
$1500 US 4 weeks
20.  Postages for a package less than 500 grams sent at room temperature by a courier company such as FedEx and TNT and dangerous goods test (DGM) for liquid materials and ice packs
$80 US each time
($65 for transport and $15 for DGM fee for liquid materials)
3-4 days

 
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