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     Mouse monoclonal antibodies
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     Antibody purification
     Peptide synthesis
     Antigen preparation
     Antibody characterization
     Antibody modification
     Antibody sequencing
Antibody sequencing
We provide sequencing service for VH and VL regions and CH and CL regions of murine monoclonal antibodies. We also provide our predictions for antibody complementary-determining regions (CDR). On request, we provide customers with plasmids containing VH and VL cDNA. The whole project takes about 4 weeks.
Work includes isolating RNA from hybridomas, trancribing into cDNA, PCRing using various primers, ligating PCR products onto a vector, transforming bacteria and finally sequencing. Sequences from ten clones will be reported.
The cost for this service is as below.
Sequencing variable (VH and VLregions
$800 US
CDR analysis
Free of charge
VH and VL inserted plasmids
$100 US
Sequencing constant (CH and CLregions       

 $300 US

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