We provide sequencing service for VH and VL regions and CH and CL regions of murine monoclonal antibodies. We also provide our predictions for antibody complementary-determining regions (CDR). On request, we provide customers with plasmids containing VH and VL cDNA. The whole project takes about 4 weeks.
Work includes isolating RNA from hybridomas, trancribing into cDNA, PCRing using various primers, ligating PCR products onto a vector, transforming bacteria and finally sequencing. Sequences from ten clones will be reported.
The cost for this service is as below.
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Work
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Cost
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Sequencing variable (VH and VL) regions
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$800 US
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CDR analysis
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Free of charge
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VH and VL inserted plasmids
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$100 US
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Sequencing constant (CH and CL) regions
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$300 US
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