Antibody digestion to Fab or F(ab')2 fragments
We use pepsin for F(ab')2 fragment production and papain for Fab fragment production. Before digesting large amount of antibodies, we perform an analytical digestion to optimize conditions. Undigested antibodies will be removed by protein A or protein G columns.
Customers should provide minimum of 10 mg antibodies. Normally 3 mg of Fab or F(ab')2 fragments can be obtained from 10 mg antibodies after absorption with Protein A.
We charge $100 US for analytical digestion by pepsin and $150 for analytical digestion by papain. We charge $450 US for digestion of 10 mg antibody by pepsin and $550 US for digestion of 10 mg antibody by papain.
Absea provides peroxidase, FITC and biotin conjugation on antibody.
We charge $100 US for labeling FITC or biotin onto 5 mg of antibodies. We charge $300 US for labeling peroxidase onto 5 mg of antibodies.