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Antibody characterization
  •  Selection of antibodies for dot blot, Western blotting and flow cytometry
  •  Selection of capture and detection antibody pairs for sandwich ELISA

Selection of antibodies for dot blot, Western blotting and flow cytometry
In monoclonal antibody production, positive cells are normally selected by ELISA. If customers require, we can go further to check antibody reactivates in dot blot, Western blotting and flow cytometry.
 
For dot blot, we charge 15 USD for each clone for testing up to 6 antigens.
For Western blot, we charge 10 USD for each clone against one antigen.
For flow cytometry, providing cells are available, we charge 35 USD for each clone using FITC conjugated secondary antibody.
 
Selection of capture and detection antibody pairs for sandwich ELISA
Usually several monoclonal antibodies with different epitope specificities can be generated against a single antigen. Some of them have epitopes far apart so that they do not compete each other on binding of the antigen. Such pairs can be used for the quantitative detection of antigens in solution by sandwich ELISA, one acts as capture antibody and the other labeled with peroxidase acts as detection antibody.
 
Customers who require this service please contact us.
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