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     Mouse monoclonal antibodies
     Rat monoclonal antibodies
     Polyclonal antibodies
     Antibody purification
     Peptide synthesis
     Antigen preparation
     Antibody characterization
     Antibody modification
     Antibody sequencing
Antigen preparation
  • Antigens provided by customers
  • Antigens expressed by E. coli
  • Coupling peptides onto carrier proteins

Antigens provided by customers
Antigens can be peptides cross-linked on to carrier proteins, protein antigens or fusion proteins. They should be soluble, not toxic, not infectious and at least 80% pure judged by SDS-PAGE. Molecular mass of antigens should preferably be over 20 kDa.
The amount of antigens required is listed below.
Antibodies to be generated
Amount required
Rat monoclonals
1.5 mg
Murine monoclonals
1 mg
Rat polyclonals
0.3 mg
Rabbit polyclonals
2 mg
Antigens expressed by E. coli
We offer antigen expression services for customers who provide us with cDNA inserted into expression vector for E. coli. Please note that customers should do test expression and know the protein can be expressed. If customers have not done test expression, we may do this for customers.
We charge $500 US for 2-3 mg antigen expressed, which is sufficient for most monoclonal and polyclonal antibody production. Each of 3 mg additional antigen expression costs $200 US. Work includes transformation of the plasmid into E. coli, expression and purification of the antigen, SDS-PAGE of the purified antigen. In the case that we do test expression for customers, if the project is terminated because the protein can not be expressed or expressed too little to be purified, we shall still charge the customer $150.
Coupling peptides onto carrier proteins
We use bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH) or Pierce Blue Carrier as carrier proteins. Peptide should contain a cysteine residue. We charge $100 US for coupling 2-5 mg peptide onto carrier protein.
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