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     Mouse monoclonal antibodies
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Antibody purification
We provide antibody purification service for customers we have made monoclonal antibodies or polyclonal anti-sera.

Murine and rat IgG monoclonal antibodies from tissue culture supernatant are purified using protein A or protein G columns. Murine and rat IgA and IgM monoclonal antibodies from tissue culture supernatants are affinity purified using antigen coupled Sepharose beads. Polyclonal antibodies are affinity purified from anti-sera using antigen coupled Sepharose beads. The prices are listed as below.
 
Table 1. Antibody purification price
Work
Price
Time taken
1. Purification of 5 mg IgG monoclonal antibody from culture supernatant.  
 
Work includes cell culture, antibody purification by protein G or protein A columns, ELISA and SDS-PAGE for purified antibody.
$400 US
About 3 weeks
2. Purification of 5 mg IgA or IgM monoclonal antibody from culture supernatant.  
 
Work includes cell culture, preparation of antigen coupled Sepharose beads or using protein L beads, antibody purification, ELISA and SDS-PAGE for purified antibody.
$600 US
About 3 weeks
3. Affinity purification of 5 mg polyclonal antibody from antiserum.
 
Work includes preparation of antigen coupled Sepharose beads, antibody affinity purification, ELISA and SDS-PAGE for purified antibody.
$400 US
One week
4. Tandem affinity purification using two antigen columns to purify polyclonal antibody from antiserum
 
Work includes preparation of two antigen coupled Sepharose beads, tandem antibody affinity purification, ELISA and SDS-PAGE for purified antibody.
$600 US
One week
 
 
 
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