We make rabbit or rat polyclonal antibodies to peptides, protein antigens or fusion proteins. We also provide antigen preparation services that include cDNA expression, peptide design, peptide synthesis and cross-linking peptides onto carrier proteins. On request, we shall further affinity purify antibodies.
Producing polyclonal anti-serum normally takes 6-8 weeks. Antibody titre in serum is checked by ELISA. If the animal has responded poorly, we shall sacrifice it and make polyclonal anti-serum using another rabbit or rat free of charge. If both animals respond poorly, the protein should be not immunogenic and we shall not try more.
We charge $300 US for 1-4.5 ml rat anti-serum and $400 US for a minimum of 35 ml rabbit anti-serum. Customers should pay within 30 days after receiving an invoice from Absea. Customers still need to pay two third cost If a project has failed.
On request, we can do more boosts on rabbits in 2-3 weeks intervals and each time take 15 ml blood (7-8 ml serum) and do ELISA for it. Each additional boost costs $100.